Description: Recombinant human PD-L1/CD274, extracellular domain is produced in E. coli. The final protein sequence contains F19-H240 of human PD-L1 fused to a polyhistidine tag on the carboxyl terminus.

Background: PD-L1 is a ligand for program cell death 1 (PD1). PD-L1 suppresses immune response against tumor cells upon binding to PD1 receptor. PD-L1 is expressed on T and B cells, macrophages, dendritic cells, and some nonimmune cells. Binding of PD-L1 to its receptor, PD1 on the surface of T cells resultes in deactivation of T cells. Blockade of PD-1 or PD-L1 by specific antibodies leads to enhanced antitumor immunity of T cells.

Sequence :MPGFTVTVPK DLYVVEYGSN MTIECKFPVE KQLDLAALIV YWEMEDKNII QFVHGEEDLK VQHSSYRQRA RLLKDQLSLG NAALQITDVK LQDAGVYRCM ISYGGADYKR ITVKVNAPYN KINQRILVVD PVTSEHELTC QAEGYPKAEV IWTSSDHQVL SGKTTTTNSK REEKLFNVTS TLRINTTTNE IFYCTFRRLD PEENHTAELV IPELPLAHPP NERTHHHHHH

Accession #: Q9NZQ7

Quality control: Verified by disulfide mapping and Mass Spectrometry analyses.

Product Source: PD-L1 was produced in E. coli cells transformed with the coding sequence of the extracellular domain of human PD-L1 gene.

Usage: FOR LABORATORY RESEARCH USE ONLY.

References:

1. Hamanishi, Junzo, et al. "Programmed cell death 1 ligand 1 and tumor-infiltrating CD8+ T lymphocytes are prognostic factors of human      ovarian cancer." Proceedings      of the National       Academy of Sciences 104.9 (2007): 3360-3365.

2. Lin, David Yin-wei, et al. "The PD-1/PD-L1 complex resembles the antigen-binding Fv domains of antibodies and T cell receptors." Proceedings of the National Academy of Sciences 105.8 (2008): 3011-3016.